Microtomy is a technique used in histology studies of human tissue for diagnostic and therapeutic purposes that allows a morphological study of tissues and organs, which may include a biopsy (taking the tissue sample) or an evaluation of the material obtained during surgery. It is an important method to detect cancer, as well as to determine the effectiveness of treatments [1]. The microtome is used for cutting paraffin blocks containing fragments of organs, with a thickness varying from 5 to 15 μm. These very thin samples become optically transparent under the microscope, making it possible to study the sample. There are several types of microtomes on the market, with different manual actuations, but mostly cyclic motion [2]. The frequent use of these devices leads to continuous repetition of movements and to postures that, in the long term, may cause musculoskeletal injuries [3]. The study of work-related injuries is generally done by inquiries [4, 5]. New works tried to incorporate visual observations [3] or surface electromyography in the research of ergonomic related injuries [6]. The use of video allows for an analysis of the posture of the subject that is latter compared with the RULA ergonomic assessment to evaluate the potential for injuries [7]. The characterization of movement using kinematics is most useful to understand the motion and its important phases or positions. It is used in different areas to support decision making and injury potential [8–10]. The fundamental objective of this work is to identify potential correlations to injury risk of the motion performed when using a microtome. To complete the objective, the kinematics of the movement associated with the manipulation of a hybrid microtome is obtained in a laboratory setting. The identification of key moments of the motion, such as high horizontal velocities and accelerations obtained at the elbow and wrist, is important to assess possible injury risks associated with the manipulation of this type of equipment by using the available published information.